The Lineup take you now behind the scenes of a police headquarters in a great American city where under the cold, glaring lights will pass before you the innocent, the vagrant, the thief, the murderer.

Lieutenant Ben Guthrie working on a case in which four young men held-up a theater. Later the four men hold up the Lincoln theater and a police officer and one of the hold-up men are shot and injured, the hold-up man later dies. Upon leaving the hospital Lieutenant Guthrie is kidnapped by the other three hold-up men.